SUPT20H Antibody

ProSci
Product Code: PSI-22-898
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-898-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SUPT20H antibody (22-898) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human colon using SUPT20H antibody (22-898) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using SUPT20H antibody (22-898) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using SUPT20H antibody (22-898) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of A549 cells using SUPT20H antibody (22-898). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SUPT20H antibody (22-898) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using SUPT20H antibody (22-898) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using SUPT20H antibody (22-898) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using SUPT20H antibody (22-898) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using SUPT20H antibody (22-898). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
C13, C13orf19, FAM48A, FP757, P38IP, SPT2transcription factor SPT20 homolog, family with sequence similarity 48, member A, protein FAM48A, suppressor of Ty 20 homolog, transcription factor (p38 interacting protein), tumor rejection antigen
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 580-779 of human SUPT20H (NP_001014308.2).
NCBI Gene ID #:
55578
NCBI Official Name:
SPT20 homolog, SAGA complex component
NCBI Official Symbol:
SUPT20H
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 86kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
Q8NEM7
User NOte:
Optimal dilutions for each application to be determined by the researcher.