PPAN Antibody

ProSci
Product Code: PSI-22-900
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-900-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PPAN Antibody (22-900) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded human prostate using PPAN antibody (22-900) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using PPAN antibody (22-900) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of A549 cells using PPAN antibody (22-900).

Western blot analysis of extracts of various cell lines, using PPAN Antibody (22-900) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using PPAN antibody (22-900) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PPAN antibody (22-900) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A549 cells using PPAN antibody (22-900).

Further Information

Additional Names:
Suppressor of SWI4 1 homolog, Ssf-1, Brix domain-containing protein 3, Peter Pan homolog, PPAN, BXDC3, SSF1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PPAN (NP_064615.3).
NCBI Gene ID #:
56342
NCBI Official Name:
Suppressor of SWI4 1 homolog
NCBI Official Symbol:
PPAN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 53kDa/55kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9NQ55
User NOte:
Optimal dilutions for each application to be determined by the researcher.