NAA60 Antibody

ProSci
Product Code: PSI-22-906
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-906-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NAA60 antibody (22-906) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver cancer using NAA60 antibody (22-906) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using NAA60 antibody (22-906) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric using NAA60 antibody (22-906) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using NAA60 antibody (22-906) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using NAA60 antibody (22-906) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using NAA60 antibody (22-906) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric using NAA60 antibody (22-906) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
N-alpha-acetyltransferase 60, Histone acetyltransferase type B protein 4, HAT4, N-acetyltransferase 15, NatF catalytic subunit, NAA60, HAT4, NAT15
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human NAA60 (NP_079121.1).
NCBI Gene ID #:
79903
NCBI Official Name:
N-alpha-acetyltransferase 60
NCBI Official Symbol:
NAA60
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9H7X0
User NOte:
Optimal dilutions for each application to be determined by the researcher.