RASSF5 Antibody

ProSci
Product Code: PSI-22-908
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-908-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RASSF5 antibody (22-908) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunohistochemistry of paraffin-embedded human prostate using RASSF5 antibody (22-908) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using RASSF5 antibody (22-908) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using RASSF5 antibody (22-908) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
NORE1, RAPL, Ras association domain-containing protein 5, New ras effector 1, Maxp1, NORE1A, NORE1B, RASSF3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human RASSF5 (NP_872606.1).
NCBI Gene ID #:
83593
NCBI Official Name:
Ras association (RalGDS/AF-6) domain family member 5
NCBI Official Symbol:
RASSF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa, 45kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
Q8WWW0
User NOte:
Optimal dilutions for each application to be determined by the researcher.