BCAT2 Antibody

ProSci
Product Code: PSI-22-929
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-929-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using BCAT2 Antibody (22-929) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Immunofluorescence analysis of NIH-3T3 cells using BCAT2 antibody (22-929) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using BCAT2 Antibody (22-929) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunofluorescence analysis of NIH-3T3 cells using BCAT2 antibody (22-929) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Branched-chain-amino-acid aminotransferase, mitochondrial, BCAT(m), Placental protein 18, PP18, BCAT2, BCATM, BCT2, ECA40
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human BCAT2 (NP_001181.2).
NCBI Gene ID #:
587
NCBI Official Name:
Branched-chain-amino-acid aminotransferase, mitochondrial
NCBI Official Symbol:
BCAT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
O15382
User NOte:
Optimal dilutions for each application to be determined by the researcher.