DFNA5 Antibody

ProSci
Product Code: PSI-22-935
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-935-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DFNA5 Antibody (22-935) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human liver damage using DFNA5 antibody (22-935) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using DFNA5 antibody (22-935) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using DFNA5 antibody (22-935) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using DFNA5 antibody (22-935) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using DFNA5 Antibody (22-935) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver damage using DFNA5 antibody (22-935) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DFNA5 antibody (22-935) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using DFNA5 antibody (22-935) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DFNA5 antibody (22-935) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
DFNA5, ICERE-1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DFNA5 (NP_004394.1).
NCBI Gene ID #:
1687
NCBI Official Name:
deafness, autosomal dominant 5
NCBI Official Symbol:
DFNA5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
O60443
User NOte:
Optimal dilutions for each application to be determined by the researcher.