PIP4K2A Antibody

ProSci
Product Code: PSI-22-946
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-946-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PIP4K2A antibody (22-946) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded rat kidney using PIP4K2A antibody (22-946) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using PIP4K2A antibody (22-946) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using PIP4K2A antibody (22-946).

Western blot analysis of extracts of various cell lines, using PIP4K2A antibody (22-946) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using PIP4K2A antibody (22-946) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using PIP4K2A antibody (22-946) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PIP4K2A antibody (22-946).

Further Information

Additional Names:
PIP4K2A, PI(5)P 4-kinase type II alpha, PIP5KII-alpha, PIP5KIII, PIPK, PtdIns(4)P-5-kinase B isoform, PtdIns(4)P-5-kinase C isoform, PI5P4KA, PIP4KII-alpha, PIP5K2, PIP5KIIalpha, PIP5K2A, PIP5KIIA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 120-350 of human PIP4K2A (NP_005019.2).
NCBI Gene ID #:
5305
NCBI Official Name:
phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
NCBI Official Symbol:
PIP4K2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P48426
User NOte:
Optimal dilutions for each application to be determined by the researcher.