TEAD3 Antibody

ProSci
Product Code: PSI-22-951
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-951-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TEAD3 antibody (22-951) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human colon using TEAD3 antibody (22-951) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of A549 cells using TEAD3 antibody (22-951).

Western blot analysis of extracts of various cell lines, using TEAD3 antibody (22-951) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using TEAD3 antibody (22-951) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using TEAD3 antibody (22-951).

Further Information

Additional Names:
TEAD3, TEF5, TEAD5, TEF-5, DTEF-1, ETFR-1, TEAD-3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is predominantly expressed in the placenta and is involved in the transactivation of the chorionic somatomammotropin-B gene enhancer. Translation of this protein is initiated at a non-AUG (AUA) start codon.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 130-330 of human TEAD3 (NP_003205.2).
NCBI Gene ID #:
7005
NCBI Official Name:
TEA domain family member 3
NCBI Official Symbol:
TEAD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 49kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Transcription
Swissprot #:
Q99594
User NOte:
Optimal dilutions for each application to be determined by the researcher.