ALDH1A2 Antibody

ProSci
Product Code: PSI-22-990
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-990-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ALDH1A2 antibody (22-990) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using ALDH1A2 antibody (22-990) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using ALDH1A2 antibody (22-990) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using ALDH1A2 antibody (22-990) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ALDH1A2 antibody (22-990) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using ALDH1A2 antibody (22-990) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using ALDH1A2 antibody (22-990) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ALDH1A2 antibody (22-990) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
RALDH(II), RALDH2, RALDH2-T, retinal dehydrogenase 2, RALDH 2, retinaldehyde-specific dehydrogenase type 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALDH1A2 (NP_733797.1).
NCBI Gene ID #:
8854
NCBI Official Name:
aldehyde dehydrogenase 1 family member A2
NCBI Official Symbol:
ALDH1A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
O94788
User NOte:
Optimal dilutions for each application to be determined by the researcher.