UBA6 Antibody

ProSci
Product Code: PSI-22-995
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-995-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using UBA6 antibody (22-995) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human kidney using UBA6 antibody (22-995) at dilution of 1:100 (40x lens).
3 / 4
Immunofluorescence analysis of A549 cells using UBA6 antibody (22-995). Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug UBA6 antibody (22-995). Western blot was performed from the immunoprecipitate using UBA6 antibody (22-995) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using UBA6 antibody (22-995) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human kidney using UBA6 antibody (22-995) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using UBA6 antibody (22-995). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug UBA6 antibody (22-995). Western blot was performed from the immunoprecipitate using UBA6 antibody (22-995) at a dilition of 1:1000.

Further Information

Additional Names:
UBA6, FLJ10808, Monocyte protein 4, MOP-4, Ubiquitin-activating enzyme 6, E1-L2, MOP4, UBE1L2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UBA6 (NP_060697.4).
NCBI Gene ID #:
55236
NCBI Official Name:
ubiquitin-like modifier activating enzyme 6
NCBI Official Symbol:
UBA6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 59kDa, 118kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
A0AVT1
User NOte:
Optimal dilutions for each application to be determined by the researcher.