SMAD2/SMAD3 Antibody

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ProSci
Product Code: PSI-23-014
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-014-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HeLa cells, using SMAD2/SMAD3 antibody (23-013) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human kidney using SMAD2/SMAD3 antibody (23-013) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric using SMAD2/SMAD3 antibody (23-013) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using SMAD2/SMAD3 antibody (23-013) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of HeLa cells, using SMAD2/SMAD3 antibody (23-013) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human kidney using SMAD2/SMAD3 antibody (23-013) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric using SMAD2/SMAD3 antibody (23-013) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SMAD2/SMAD3 antibody (23-013) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human SMAD3 (NP_005893.1).
NCBI Gene ID #:
4087/4088
NCBI Official Symbol:
SMAD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56-60kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell, Transcription
Swissprot #:
P84022/Q15796
User NOte:
Optimal dilutions for each application to be determined by the researcher.