EIF4G1 Antibody

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ProSci
Product Code: PSI-23-023
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-023-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of THP-1 cells, using EIF4G1 Antibody (23-023) at 1:200 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver damage using EIF4G1 Antibody (23-023) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using EIF4G1 Antibody (23-023) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using EIF4G1 Antibody (23-023) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using EIF4G1 antibody (23-023). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of THP-1 cells, using EIF4G1 Antibody (23-023) at 1:200 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using EIF4G1 Antibody (23-023) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using EIF4G1 Antibody (23-023) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EIF4G1 Antibody (23-023) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF4G1 antibody (23-023). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
P220, EIF4F, EIF4G, EIF4GI, PARK18, EIF-4G1, OCT3, OCT4, OTF3,
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).
NCBI Gene ID #:
1981
NCBI Official Name:
eukaryotic translation initiation factor 4 gamma, 1
NCBI Official Symbol:
EIF4G1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 230kDa
Purification:
Affinity purification
Research Area:
Immunology, Signal Transduction
Swissprot #:
Q04637
User NOte:
Optimal dilutions for each application to be determined by the researcher.