C1GALT1C1 Antibody

ProSci
Product Code: PSI-23-046
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-046-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using C1GALT1C1 antibody (23-046) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using C1GALT1C1 antibody (23-046) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using C1GALT1C1 antibody (23-046) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using C1GALT1C1 antibody (23-046) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using C1GALT1C1 antibody (23-046) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using C1GALT1C1 antibody (23-046) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
C1GALT2, C38H2-L1, COSMC, HSPC067, MST143, TNPS, C1GALT1-specific chaperone 1, C38H2-like protein 1, beta 1,3-galactosyltransferase 2, core 1 beta1,3-galactosyltransferase 2, core 1 beta3-Gal-T2, core 1 beta3-galactosyltransferase-specific molecular chaperone
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes a type II transmembrane protein that is similar to the core 1 beta1,3-galactosyltransferase 1, which catalyzes the synthesis of the core-1 structure, also known as Thomsen-Friedenreich antigen, on O-linked glycans. This gene product lacks the galactosyltransferase activity itself, but instead acts as a molecular chaperone required for the folding, stability and full activity of the core 1 beta1,3-galactosyltransferase 1. Mutations in this gene have been associated with Tn syndrome. Alternatively spliced transcript variants encoding the same protein have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-190 of human C1GALT1C1 (NP_689905.1).
NCBI Gene ID #:
29071
NCBI Official Name:
C1GALT1 specific chaperone 1
NCBI Official Symbol:
C1GALT1C1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q96EU7
User NOte:
Optimal dilutions for each application to be determined by the researcher.