ITGA2 Antibody, KO Validated

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ProSci
Product Code: PSI-23-063
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-063-50uL50uL£505.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ITGA2 antibody (23-063) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Western blot analysis of extracts from normal (control) and ITGA2 knockout (KO) HeLa cells, using ITGA2 antibody (23-063) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody (23-063) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody (23-063) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using ITGA2 antibody (23-063) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ITGA2 antibody (23-063) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Western blot analysis of extracts from normal (control) and ITGA2 knockout (KO) HeLa cells, using ITGA2 antibody (23-063) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody (23-063) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody (23-063) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using ITGA2 antibody (23-063) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ITGA2, Alpha2 integrin, BR, CD49b antigen, CD49B, Integrin alpha2, Integrin Alpha 2, Integrin alpha-2, HPA-5, VLA-2, VLAA2, VLA-2 subunit alpha, Collagen receptor, GPIa, Platelet antigen Br, Platelet glycoprotein GPIa, VLA2 receptor, alpha-2 subunit
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human ITGA2
NCBI Gene ID #:
3673
NCBI Official Name:
integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
NCBI Official Symbol:
ITGA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 150kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P17301
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.