NOTCH1 Antibody, KO Validated

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ProSci
Product Code: PSI-23-069
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-069-50uL50uL£505.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NOTCH1 antibody (23-069) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 4
Western blot analysis of extracts from normal (control) and NOTCH1 knockout (KO) HeLa cells, using NOTCH1 antibody (23-069) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat spleen using NOTCH1 antibody (23-069) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using NOTCH1 antibody (23-069) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using NOTCH1 antibody (23-069) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts from normal (control) and NOTCH1 knockout (KO) HeLa cells, using NOTCH1 antibody (23-069) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spleen using NOTCH1 antibody (23-069) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NOTCH1 antibody (23-069) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
NOTCH1, TAN1, hN1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human NOTCH1
NCBI Gene ID #:
4851
NCBI Official Name:
notch 1
NCBI Official Symbol:
NOTCH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 300kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction, Stem Cell, Transcription
Swissprot #:
P46531
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.