GLUT4 Antibody

ProSci
Product Code: PSI-23-070
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-070-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GLUT4 antibody (23-070) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse heart using GLUT4 antibody (23-070) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using GLUT4 antibody (23-070) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using GLUT4 antibody (23-070) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using GLUT4 antibody (23-070) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse heart using GLUT4 antibody (23-070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using GLUT4 antibody (23-070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using GLUT4 antibody (23-070) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
SLC2A4, solute carrier family 2 (facilitated glucose transporter), member 4, insulin-responsive glucose transporter type 4, GLUT4, glucose transporter 4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human GLUT4 (NP_001033.1).
NCBI Gene ID #:
6517
NCBI Official Name:
solute carrier family 2 (facilitated glucose transporter), member 4
NCBI Official Symbol:
SLC2A4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 54kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P14672
User NOte:
Optimal dilutions for each application to be determined by the researcher.