TXN Antibody

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ProSci
Product Code: PSI-23-071
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-071-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TXN antibody (23-071) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse lung using TXN antibody (23-071) at dilution of 1:100 (40x lens).
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Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug TXN antibody (23-071). Western blot was performed from the immunoprecipitate using TXN antibody (23-071) at a dilition of 1:500.
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Western blot analysis of extracts of various cell lines, using TXN antibody (23-071) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using TXN antibody (23-071) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug TXN antibody (23-071). Western blot was performed from the immunoprecipitate using TXN antibody (23-071) at a dilition of 1:500.

Documents

Further Information

Additional Names:
TXN, ADF, ATL-derived factor, SASP, Thioredoxin, TRX1, Thioredoxin delta 3, TRX, TXN delta 3, TRDX
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 20 to the C-terminus of human TXN (NP_003320.2).
NCBI Gene ID #:
7295
NCBI Official Name:
thioredoxin
NCBI Official Symbol:
TXN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 12kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P10599
User NOte:
Optimal dilutions for each application to be determined by the researcher.