BCS1L Antibody

ProSci
Product Code: PSI-23-078
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-078-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using BCS1L antibody (23-078) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human stomach using BCS1L antibody (23-078) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat brain using BCS1L antibody (23-078) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using BCS1L antibody (23-078) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using BCS1L antibody (23-078) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using BCS1L antibody (23-078) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using BCS1L antibody (23-078) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using BCS1L antibody (23-078) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
BCS, BCS1, BJS, FLNMS, GRACILE, Hs.6719, MC3DN1, PTD, h-BCS, h-BCS1, mitochondrial chaperone BCS1, BC1 (ubiquinol-cytochrome c reductase) synthesis-like, BCS1-like protein, mitochondrial complex III assembly
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a homolog of the S. cerevisiae bcs1 protein which is involved in the assembly of complex III of the mitochondrial respiratory chain. The encoded protein does not contain a mitochondrial targeting sequence but experimental studies confirm that it is imported into mitochondria. Mutations in this gene are associated with mitochondrial complex III deficiency and the GRACILE syndrome. Several alternatively spliced transcripts encoding two different isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 33-240 of human BCS1L (NP_004319.1).
NCBI Gene ID #:
617
NCBI Official Name:
BCS1 homolog, ubiquinol-cytochrome c reductase complex chaperone
NCBI Official Symbol:
BCS1L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
Q9Y276
User NOte:
Optimal dilutions for each application to be determined by the researcher.