ARID3A Antibody

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ProSci
Product Code: PSI-23-092
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-092-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ARID3A antibody (23-092) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat ileum using ARID3A antibody (23-092) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human oophoroma using ARID3A antibody (23-092) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung cancer using ARID3A antibody (23-092) at dilution of 1:100 (40x lens).
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Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug ARID3A antibody (23-092). Western blot was performed from the immunoprecipitate using ARID3A antibody (23-092) at a dilition of 1:500.
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Western blot analysis of extracts of various cell lines, using ARID3A antibody (23-092) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ileum using ARID3A antibody (23-092) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using ARID3A antibody (23-092) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung cancer using ARID3A antibody (23-092) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug ARID3A antibody (23-092). Western blot was performed from the immunoprecipitate using ARID3A antibody (23-092) at a dilition of 1:500.

Documents

Further Information

Additional Names:
ARID3A, BRIGHT, DRIL1, DRIL3, E2FBP1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 324-593 of human ARID3A (NP_005215.1).
NCBI Gene ID #:
1820
NCBI Official Name:
AT rich interactive domain 3A (BRIGHT-like)
NCBI Official Symbol:
ARID3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 95kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q99856
User NOte:
Optimal dilutions for each application to be determined by the researcher.