ETFA Antibody

ProSci
Product Code: PSI-23-094
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-094-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ETFA antibody (23-094) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human prostate using ETFA antibody (23-094) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using ETFA antibody (23-094) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using ETFA antibody (23-094) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ETFA antibody (23-094) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using ETFA antibody (23-094) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ETFA antibody (23-094) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ETFA antibody (23-094) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
ETFA, EMA, GA2, MADD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ETFA (NP_000117.1).
NCBI Gene ID #:
2108
NCBI Official Name:
electron-transfer-flavoprotein, alpha polypeptide
NCBI Official Symbol:
ETFA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P13804
User NOte:
Optimal dilutions for each application to be determined by the researcher.