HOXD10 Antibody

ProSci
Product Code: PSI-23-106
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-106-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using HOXD10 antibody (23-106) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of U2OS cells using HOXD10 antibody (23-106) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HOXD10 antibody (23-106) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using HOXD10 antibody (23-106) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HOXD10, homeobox D10, HOX4, HOX4D, HOX4E, Hox-4.4, homeo box 4D, homeo box D10
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox D genes located on chromosome 2. The encoded nuclear protein functions as a sequence-specific transcription factor that is expressed in the developing limb buds and is involved in differentiation and limb development. Mutations in this gene have been associated with Wilm's tumor and congenital vertical talus (also known as 'rocker-bottom foot' deformity or congenital convex pes valgus) and/or a foot deformity resembling that seen in Charcot-Marie-Tooth disease.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HOXD10 (NP_002139.2).
NCBI Gene ID #:
3236
NCBI Official Name:
homeobox D10
NCBI Official Symbol:
HOXD10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
P28358
User NOte:
Optimal dilutions for each application to be determined by the researcher.