IRF1 Antibody

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ProSci
Product Code: PSI-23-112
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-112-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using IRF1 antibody (23-112) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human lung cancer using IRF1 antibody (23-112) (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using IRF1 antibody (23-112) (40x lens).
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Western blot analysis of extracts of various cell lines, using IRF1 antibody (23-112) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using IRF1 antibody (23-112) (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IRF1 antibody (23-112) (40x lens).

Documents

Further Information

Additional Names:
IRF1, IRF-1, MAR
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human IRF1
NCBI Gene ID #:
3659
NCBI Official Name:
interferon regulatory factor 1
NCBI Official Symbol:
IRF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Transcription
Swissprot #:
P10914
User NOte:
Optimal dilutions for each application to be determined by the researcher.