YBX1 Antibody, KO Validated

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ProSci
Product Code: PSI-23-119
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-119-50uL50uL£505.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HepG2 cells, using YBX1 antibody (23-119) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody (23-119) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human lung cancer using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of HeLa cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of PC-12 cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of HepG2 cells, using YBX1 antibody (23-119) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody (23-119) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human lung cancer using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
NSEP1, YB1, BP-8, CSDB, DBPB, YB-1, CSDA2, NSEP1, NSEP-1, MDR-NF1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
NCBI Gene ID #:
4904
NCBI Official Name:
Y box binding protein 1
NCBI Official Symbol:
YBX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 49kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Stem Cell, Transcription
Swissprot #:
P67809
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.