PRKDC Antibody

ProSci
Product Code: PSI-23-125
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-125-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PRKDC antibody (23-125) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PRKDC antibody (23-125) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using PRKDC antibody (23-125) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using PRKDC antibody (23-125) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U-2OS cells using PRKDC antibody (23-125) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PRKDC antibody (23-125) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PRKDC antibody (23-125) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using PRKDC antibody (23-125) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PRKDC antibody (23-125) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2OS cells using PRKDC antibody (23-125) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PRKDC, DNA-PK, DNAPK, DNPK1, HYRC, HYRC1, p350, Scid, XRCC7, DNA-PK catalytic subunit, DNA-PKcs, p460, DNA-dependent protein kinase catalytic subunit
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human PRKDC
NCBI Gene ID #:
5591
NCBI Official Name:
protein kinase, DNA-activated, catalytic polypeptide
NCBI Official Symbol:
PRKDC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 469kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P78527
User NOte:
Optimal dilutions for each application to be determined by the researcher.