RAC1 Antibody

ProSci
Product Code: PSI-23-129
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-129-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RAC1 antibody (23-129) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human gastric using RAC1 antibody (23-129) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using RAC1 antibody (23-129). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RAC1 antibody (23-129) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human gastric using RAC1 antibody (23-129) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using RAC1 antibody (23-129). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, RAC1, TC25
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RAC1 (NP_008839.2).
NCBI Gene ID #:
5879
NCBI Official Name:
Ras-related C3 botulinum toxin substrate 1
NCBI Official Symbol:
RAC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 21kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Swissprot #:
P63000
User NOte:
Optimal dilutions for each application to be determined by the researcher.