RCN2 Antibody

ProSci
Product Code: PSI-23-130
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-130-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunohistochemistry of paraffin-embedded mouse heart using RCN2 antibody (23-130) at dilution of 1:100 (40x lens).
2 / 4
Immunohistochemistry of paraffin-embedded rat heart using RCN2 antibody (23-130) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human stomach using RCN2 antibody (23-130) at dilution of 1:100 (40x lens).
4 / 4
Western blot analysis of extracts of various cell lines, using RCN2 antibody (23-130) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Immunohistochemistry of paraffin-embedded mouse heart using RCN2 antibody (23-130) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using RCN2 antibody (23-130) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RCN2 antibody (23-130) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using RCN2 antibody (23-130) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Further Information

Additional Names:
E6BP, ERC-55, ERC55, TCBP49, reticulocalbin-2, E6-binding protein, calcium-binding protein ERC-55, reticulocalbin 2, EF-hand calcium binding domain (endoplasmic reticulum calcium-binding protein, 55kD)
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. This gene maps to the same region as type 4 Bardet-Biedl syndrome, suggesting a possible causative role for this gene in the disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 23-180 of human RCN2 (NP_002893.1).
NCBI Gene ID #:
5955
NCBI Official Name:
reticulocalbin 2
NCBI Official Symbol:
RCN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
Q14257
User NOte:
Optimal dilutions for each application to be determined by the researcher.