MAP2K4 Antibody

ProSci
Product Code: PSI-23-132
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-132-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MAP2K4 antibody (23-132) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human kidney using MAP2K4 antibody (23-132) at dilution of 1:100 (40x lens).
3 / 4
Immunofluorescence analysis of U2OS cells using MAP2K4 antibody (23-132) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug MAP2K4 antibody (23-132). Western blot was performed from the immunoprecipitate using MAP2K4 antibody (23-132) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using MAP2K4 antibody (23-132) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human kidney using MAP2K4 antibody (23-132) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MAP2K4 antibody (23-132) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug MAP2K4 antibody (23-132). Western blot was performed from the immunoprecipitate using MAP2K4 antibody (23-132) at a dilition of 1:1000.

Further Information

Additional Names:
MAP2K4, JNK activating kinase 1, JNK-activated kinase 1, JNK-activating kinase 1, MAPK/ERK kinase 4, MAPKK4, JNKK1, PRKMK4, SAPKK1, SEK1, MEK4, MKK4, SAPK/ERK kinase 1, SERK1, JNKK, MAP kinase kinase 4, MAPKK 4, MEK 4, SAPK kinase 1, SAPKK-1, SKK1
Application Note:
WB: 1:1000 - 1:4000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human MAP2K4
NCBI Gene ID #:
6416
NCBI Official Name:
mitogen-activated protein kinase kinase 4
NCBI Official Symbol:
MAP2K4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
P45985
User NOte:
Optimal dilutions for each application to be determined by the researcher.