SULT2B1 Antibody

ProSci
Product Code: PSI-23-144
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-144-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SULT2B1 antibody (23-144) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver cancer using SULT2B1 antibody (23-144) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using SULT2B1 antibody (23-144) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using SULT2B1 antibody (23-144) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using SULT2B1 antibody (23-144) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using SULT2B1 antibody (23-144).

Western blot analysis of extracts of various cell lines, using SULT2B1 antibody (23-144) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using SULT2B1 antibody (23-144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SULT2B1 antibody (23-144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using SULT2B1 antibody (23-144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SULT2B1 antibody (23-144) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using SULT2B1 antibody (23-144).

Further Information

Additional Names:
SULT2B1, HSST2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-365 of human SULT2B1 (NP_814444.1).
NCBI Gene ID #:
6820
NCBI Official Name:
sulfotransferase family, cytosolic, 2B, member 1
NCBI Official Symbol:
SULT2B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 41kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
O00204
User NOte:
Optimal dilutions for each application to be determined by the researcher.