ST8SIA2 Antibody

ProSci
Product Code: PSI-23-153
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-153-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ST8SIA2 antibody (23-153) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human stomach using ST8SIA2 antibody (23-153) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using ST8SIA2 antibody (23-153) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using ST8SIA2 antibody (23-153) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ST8SIA2 antibody (23-153) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using ST8SIA2 antibody (23-153) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ST8SIA2 antibody (23-153) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ST8SIA2 antibody (23-153) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
ST8SIA2, HsT19690, MGC116854, MGC116857, SIAT8B, ST8SIA-II, STX
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a type II membrane protein that is thought to catalyze the transfer of sialic acid from CMP-sialic acid to N-linked oligosaccharides and glycoproteins. The encoded protein may be found in the Golgi apparatus and may be involved in the production of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). This protein is a member of glycosyltransferase family 29.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human ST8SIA2 (NP_006002.1).
NCBI Gene ID #:
8128
NCBI Official Name:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
NCBI Official Symbol:
ST8SIA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 42kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q92186
User NOte:
Optimal dilutions for each application to be determined by the researcher.