TTF2 Antibody

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ProSci
Product Code: PSI-23-156
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-156-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TTF2 antibody (23-156) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
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Immunofluorescence analysis of A549 cells using TTF2 antibody (23-156). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using TTF2 antibody (23-156) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of A549 cells using TTF2 antibody (23-156). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
HuF2, ZGRF6, transcription termination factor 2, F2, RNA polymerase II termination factor, human factor 2, lodestar homolog, lodestar protein, transcription release factor 2, transcription termination factor, RNA polymerase II, zinc finger, GRF-type containing 6
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTF2 (NP_003585.3).
NCBI Gene ID #:
8458
NCBI Official Name:
transcription termination factor 2
NCBI Official Symbol:
TTF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 130kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9UNY4
User NOte:
Optimal dilutions for each application to be determined by the researcher.