DGKE Antibody

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ProSci
Product Code: PSI-23-157
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-157-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunofluorescence analysis of U2OS cells using DGKE antibody (23-157) at dilution of 1:100. Blue: DAPI for nuclear staining.
2 / 4
Immunohistochemistry of paraffin-embedded mouse kidney using DGKE antibody (23-157) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human gastric using DGKE antibody (23-157) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using DGKE antibody (23-157) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunofluorescence analysis of U2OS cells using DGKE antibody (23-157) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse kidney using DGKE antibody (23-157) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric using DGKE antibody (23-157) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using DGKE antibody (23-157) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Documents

Further Information

Additional Names:
DGKE, DAGK6, DGK, DAGK5, NPHS7
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human DGKE (NP_003638.1).
NCBI Gene ID #:
8526
NCBI Official Name:
diacylglycerol kinase, epsilon 64kDa
NCBI Official Symbol:
DGKE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 64kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P52429
User NOte:
Optimal dilutions for each application to be determined by the researcher.