PLA2G4C Antibody

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ProSci
Product Code: PSI-23-158
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-158-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of THP-1 cells, using PLA2G4C antibody (23-158) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human tonsil using PLA2G4C antibody (23-158) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using PLA2G4C antibody (23-158) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of THP-1 cells, using PLA2G4C antibody (23-158) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using PLA2G4C antibody (23-158) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PLA2G4C antibody (23-158) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
PLA2G4C, phospholipase A2, group IVC (cytosolic, calcium-independent), CPLA2-gamma, DKFZp586C0423, FLJ42247, FLJ44164, cytosolic phospholipase A2 gamma, phospholipase A2, cytosolic, calcium-independent, gamma, phospholipase A2, group IVC
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a protein which is a member of the phospholipase A2 enzyme family which hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids, both of which serve as precursors in the production of signaling molecules. The encoded protein has been shown to be a calcium-independent and membrane bound enzyme. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PLA2G4C (NP_003697.2).
NCBI Gene ID #:
8605
NCBI Official Name:
phospholipase A2 group IVC
NCBI Official Symbol:
PLA2G4C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9UP65
User NOte:
Optimal dilutions for each application to be determined by the researcher.