B3GALNT1 Antibody

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ProSci
Product Code: PSI-23-159
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-159-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using B3GALNT1 antibody (23-159) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunofluorescence analysis of U2OS cells using B3GALNT1 antibody (23-159) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using B3GALNT1 antibody (23-159) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using B3GALNT1 antibody (23-159) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
B3GALT3, GLCT3, GLOB, Gb4Cer, P, P1, beta3Gal-T3, galT3, UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1, P antigen synthase, P blood group globoside, UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 3 (Globoside blood group), UDP-GalNAc:betaGlcNAc beta-1,3-galactosaminyltransferase, polypeptide 1 (Globoside blood group), UDP-N-acetylgalactosamine:globotriaosylceramide beta-1,3-N-acetylgalactosaminyltransferase, b3Gal-T3, beta-1,3-GalNAc-T1, beta-1,3-GalTase 3, beta-1,3-galactosyltransferase 3, beta-3-Gx-T3, brainiac1, galactosylgalactosylglucosylceramide beta-D-acetyl-galactosaminyltransferase, globoside synthase, globotriaosylceramide 3-beta-N-acetylgalactosaminyltransferase
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). The encoded protein of this gene does not use N-acetylglucosamine as an acceptor sugar at all.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 44-331 of human B3GALNT1 (NP_003772.1).
NCBI Gene ID #:
8706
NCBI Official Name:
beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
NCBI Official Symbol:
B3GALNT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O75752
User NOte:
Optimal dilutions for each application to be determined by the researcher.