SQSTM1 / p62 Antibody

ProSci
Product Code: PSI-23-162
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-162-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SQSTM1 / p62 antibody (23-162) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat brain using SQSTM1 / p62 antibody (23-162) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using SQSTM1 / p62 antibody (23-162) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug SQSTM1 / p62 antibody (23-162). Western blot was performed from the immunoprecipitate using SQSTM1 / p62 antibody (23-162) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using SQSTM1 / p62 antibody (23-162) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using SQSTM1 / p62 antibody (23-162) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SQSTM1 / p62 antibody (23-162) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug SQSTM1 / p62 antibody (23-162). Western blot was performed from the immunoprecipitate using SQSTM1 / p62 antibody (23-162) at a dilition of 1:1000.

Further Information

Additional Names:
SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, ORCA, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1).
NCBI Gene ID #:
8878
NCBI Official Name:
sequestosome 1
NCBI Official Symbol:
SQSTM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Autophagy, Cell Cycle, Signal Transduction
Swissprot #:
Q13501
User NOte:
Optimal dilutions for each application to be determined by the researcher.