FBXW11 Antibody

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ProSci
Product Code: PSI-23-181
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-181-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using FBXW11 antibody (23-181) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse lung using FBXW11 antibody (23-181) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using FBXW11 antibody (23-181).
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Western blot analysis of extracts of various cell lines, using FBXW11 antibody (23-181) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using FBXW11 antibody (23-181) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using FBXW11 antibody (23-181).

Documents

Further Information

Additional Names:
FBXW11, BTRC2, BTRCP2, FBW1B, FBXW1B, Fbw11, Hos, KIAA0696
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human FBXW11 (NP_387448.2).
NCBI Gene ID #:
23291
NCBI Official Name:
F-box and WD repeat domain containing 11
NCBI Official Symbol:
FBXW11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 62kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9UKB1
User NOte:
Optimal dilutions for each application to be determined by the researcher.