HARS2 Antibody

ProSci
Product Code: PSI-23-182
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-182-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HARS2 antibody (23-182) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat brain using HARS2 antibody (23-182) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using HARS2 antibody (23-182) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using HARS2 antibody (23-182) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using HARS2 antibody (23-182) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HARS2 antibody (23-182) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
HARSL, HARSR, HO3, HisRS, PRLTS2, probable histidine--tRNA ligase, mitochondrial, HARS-related, histidine tRNA ligase 2, mitochondrial (putative), histidine translase, histidine-tRNA ligase homolog, histidyl-tRNA synthetase 2, mitochondrial (putative), histidyl-tRNA synthetase 2, mitochondrial, hisRS, probable histidyl-tRNA synthetase, mitochondrial
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 217-506 of human HARS2 (NP_036340.1).
NCBI Gene ID #:
23438
NCBI Official Name:
histidyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol:
HARS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P49590
User NOte:
Optimal dilutions for each application to be determined by the researcher.