SUZ12 Antibody, KO Validated

ProSci
Product Code: PSI-23-183
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-183-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SUZ12 Antibody (23-183) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using SUZ12 antibody (23-183) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat lung using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using SUZ12 Polyclonal Antibody (23-183) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA1 Antibody (22-980) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using SUZ12 Antibody (23-183) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using SUZ12 antibody (23-183) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SUZ12 antibody (23-183) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using SUZ12 Polyclonal Antibody (23-183) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA1 Antibody (22-980) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
SUZ12 Antibody: CHET9, JJAZ1, CHET9, KIAA0160, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, ChET 9 protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2).
NCBI Gene ID #:
23512
NCBI Official Name:
SUZ12 polycomb repressive complex 2 subunit
NCBI Official Symbol:
SUZ12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 83kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q15022
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.