RASGRP3 Antibody

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ProSci
Product Code: PSI-23-186
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-186-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RASGRP3 antibody (23-186) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of U2OS cells using RASGRP3 antibody (23-186) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using RASGRP3 antibody (23-186) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using RASGRP3 antibody (23-186) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
GRP3, ras guanyl-releasing protein 3, CalDAG-GEFIII, RAS guanyl releasing protein 3 (calcium and DAG-regulated), calcium and DAG-regulated guanine nucleotide exchange factor III, calcium- and diacylglycerol-regulated guanine nucleotide exchange factor III, guanine nucleotide exchange factor for Rap1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 540-689 of human RASGRP3 (NP_056191.1).
NCBI Gene ID #:
25780
NCBI Official Name:
RAS guanyl releasing protein 3
NCBI Official Symbol:
RASGRP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 78kDa
Purification:
Affinity purification
Research Area:
Immunology, Signal Transduction
Swissprot #:
Q8IV61
User NOte:
Optimal dilutions for each application to be determined by the researcher.