SAMHD1 Antibody, KO Validated

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ProSci
Product Code: PSI-23-189
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-189-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (23-189) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Western blot analysis of extracts from normal (control) and SAMHD1 knockout (KO) HeLa cells, using SAMHD1 antibody (23-189) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
3 / 3
Immunofluorescence analysis of A549 cells using SAMHD1 antibody (23-189). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (23-189) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SAMHD1 knockout (KO) HeLa cells, using SAMHD1 antibody (23-189) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
Immunofluorescence analysis of A549 cells using SAMHD1 antibody (23-189). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
SAM domain and HD domain 1, DCIP, CHBL2, HDDC1, MOP-5, SBBI88
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 387-626 of human SAMHD1 (NP_056289.2).
NCBI Gene ID #:
25939
NCBI Official Name:
SAM domain and HD domain 1
NCBI Official Symbol:
SAMHD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 72kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9Y3Z3
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.