INPP5K Antibody

ProSci
Product Code: PSI-23-196
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-196-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using INPP5K antibody (23-196) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human stomach using INPP5K antibody (23-196) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using INPP5K antibody (23-196) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using INPP5K antibody (23-196) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using INPP5K antibody (23-196) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using INPP5K antibody (23-196) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using INPP5K antibody (23-196) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using INPP5K antibody (23-196) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using INPP5K antibody (23-196) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using INPP5K antibody (23-196) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
MDCCAID, PPS, SKIP, inositol polyphosphate 5-phosphatase K, skeletal muscle and kidney-enriched inositol phosphatase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternatively spliced transcript variants encoding different isoforms have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 149-448 of human INPP5K (NP_057616.2).
NCBI Gene ID #:
51763
NCBI Official Name:
inositol polyphosphate-5-phosphatase K
NCBI Official Symbol:
INPP5K
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 51kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9BT40
User NOte:
Optimal dilutions for each application to be determined by the researcher.