CALML5 Antibody

ProSci
Product Code: PSI-23-197
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-197-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CALML5 antibody (23-197) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse brain using CALML5 antibody (23-197) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using CALML5 antibody (23-197) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CALML5 antibody (23-197) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using CALML5 antibody (23-197) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using CALML5 antibody (23-197) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CLSP, calmodulin-like protein 5, calmodulin-like skin protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-146 of human CALML5 (NP_059118.2).
NCBI Gene ID #:
51806
NCBI Official Name:
calmodulin like 5
NCBI Official Symbol:
CALML5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q9NZT1
User NOte:
Optimal dilutions for each application to be determined by the researcher.