DARS2 Antibody

ProSci
Product Code: PSI-23-202
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-202-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DARS2 antibody (23-202) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded human liver damage using DARS2 antibody (23-202) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using DARS2 antibody (23-202) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human stomach using DARS2 antibody (23-202) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using DARS2 antibody (23-202) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using DARS2 antibody (23-202) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DARS2 antibody (23-202) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DARS2 antibody (23-202) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
ASPRS, LBSL, MT-ASPRS, mtAspRS, aspartate--tRNA ligase, mitochondrial, aspartate tRNA ligase 2, mitochondrial, aspartyl-tRNA synthetase, mitochondrial
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 406-645 of human DARS2 (NP_060592.2).
NCBI Gene ID #:
55157
NCBI Official Name:
aspartyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol:
DARS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 74kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q6PI48
User NOte:
Optimal dilutions for each application to be determined by the researcher.