ALG1 Antibody

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ProSci
Product Code: PSI-23-205
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-205-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ALG1 antibody (23-205) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunofluorescence analysis of A549 cells using ALG1 antibody (23-205). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ALG1 antibody (23-205) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of A549 cells using ALG1 antibody (23-205). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ALG1, HMAT1, HMT-1, HMT1, MT-1, CDG1K, Mat-1, hMat-1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 215-464 of human ALG1 (NP_061982.3).
NCBI Gene ID #:
56052
NCBI Official Name:
ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase
NCBI Official Symbol:
ALG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q9BT22
User NOte:
Optimal dilutions for each application to be determined by the researcher.