RUFY1 Antibody

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ProSci
Product Code: PSI-23-217
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-217-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunohistochemistry of paraffin-embedded rat brain using RUFY1 antibody (23-217) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using RUFY1 antibody (23-217) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using RUFY1 antibody (23-217) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using RUFY1 antibody (23-217) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.

Documents

Further Information

Additional Names:
zinc finger FYVE, rab4-interacting protein, RUN and FYVE domain-containing protein 1, RUN and FYVE domain-containing 1, OTTHUMP00000223250, FYVE-finger protein EIP1, ZFYVE12, RABIP4, FLJ22251, RUN and FYVE domain containing 1, RUFY1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 439-708 of human RUFY1 (NP_079434.3).
NCBI Gene ID #:
80230
NCBI Official Name:
RUN and FYVE domain containing 1
NCBI Official Symbol:
RUFY1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q96T51
User NOte:
Optimal dilutions for each application to be determined by the researcher.