HAUS8 Antibody

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ProSci
Product Code: PSI-23-225
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-225-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HAUS8 antibody (23-225) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of U2OS cells using HAUS8 antibody (23-225) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using HAUS8 antibody (23-225) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using HAUS8 antibody (23-225) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48, HAUS8, HICE1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 166-410 of human HAUS8 (NP_219485.1).
NCBI Gene ID #:
93323
NCBI Official Name:
HAUS augmin-like complex subunit 8
NCBI Official Symbol:
HAUS8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 41kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology
Swissprot #:
Q9BT25
User NOte:
Optimal dilutions for each application to be determined by the researcher.