NDUFS7 Antibody

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ProSci
Product Code: PSI-23-234
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-23-234-50uL50uL£433.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse skeletal muscle, using NDUFS7 antibody (23-234) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human gastric cancer using NDUFS7 antibody (23-234) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using NDUFS7 antibody (23-234) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using NDUFS7 antibody (23-234) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of mouse skeletal muscle, using NDUFS7 antibody (23-234) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human gastric cancer using NDUFS7 antibody (23-234) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NDUFS7 antibody (23-234) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NDUFS7 antibody (23-234) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
NDUFS7, PSST, NADH-ubiquinon, NADH-coenzyme Q reductase, NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial, NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase), MY017, MGC120002, FLJ46880, FLJ45860, CI-20KD, CI-20
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a protein that is a subunit of one of the complexes that forms the mitochondrial respiratory chain. This protein is one of over 40 subunits found in complex I, the nicotinamide adenine dinucleotide (NADH):ubiquinone oxidoreductase. This complex functions in the transfer of electrons from NADH to the respiratory chain, and ubiquinone is believed to be the immediate electron acceptor for the enzyme. Mutations in this gene cause Leigh syndrome due to mitochondrial complex I deficiency, a severe neurological disorder that results in bilaterally symmetrical necrotic lesions in subcortical brain regions.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 39-213 of human NDUFS7 (NP_077718.3).
NCBI Gene ID #:
374291
NCBI Official Name:
NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase)
NCBI Official Symbol:
NDUFS7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 24kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
O75251
User NOte:
Optimal dilutions for each application to be determined by the researcher.