AARS Antibody

ProSci
Product Code: PSI-23-235
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-235-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using AARS antibody (23-235) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human liver damage using AARS antibody (23-235) (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded rat kidney using AARS antibody (23-235) (40x lens).

Western blot analysis of extracts of various cell lines, using AARS antibody (23-235) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using AARS antibody (23-235) (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using AARS antibody (23-235) (40x lens).

Further Information

Additional Names:
AARS, CMT2N
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human AARS
NCBI Gene ID #:
16
NCBI Official Name:
alanyl-tRNA synthetase
NCBI Official Symbol:
AARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 107kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P49588
User NOte:
Optimal dilutions for each application to be determined by the researcher.