ADAR Antibody

ProSci
Product Code: PSI-23-240
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-240-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ADAR antibody (23-240) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
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Immunohistochemistry of paraffin-embedded rat testis using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
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Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SUZ12 antibody (23-183). Western blot was performed from the immunoprecipitate using SUZ12 antibody (23-183) at a dilition of 1:500.
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Immunofluorescence analysis of HeLa cells using ADAR antibody (23-240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ADAR antibody (23-240) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat testis using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ADAR antibody (23-240) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SUZ12 antibody (23-183). Western blot was performed from the immunoprecipitate using SUZ12 antibody (23-183) at a dilition of 1:500.
Immunofluorescence analysis of HeLa cells using ADAR antibody (23-240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136, double-stranded RNA-specific adenosine deaminase, 136 kDa double-stranded RNA-binding protein, adenosine deaminase acting on RNA 1-A, dsRNA adenosine deaminase, dsRNA adeonosine deaminase, interferon-induced protein 4, interferon-inducible protein 4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ADAR (NP_001102.2).
NCBI Gene ID #:
103
NCBI Official Name:
adenosine deaminase RNA specific
NCBI Official Symbol:
ADAR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 136kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P55265
User NOte:
Optimal dilutions for each application to be determined by the researcher.