ANGPT1 Antibody

ProSci
Product Code: PSI-23-247
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-247-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ANGPT1 antibody (23-247) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded rat lung using ANGPT1 antibody (23-247) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using ANGPT1 antibody (23-247) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using ANGPT1 antibody (23-247) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ANGPT1 antibody (23-247) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using ANGPT1 antibody (23-247) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using ANGPT1 antibody (23-247) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ANGPT1 antibody (23-247) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
ANGPT1, angiopoietin 1, ANG1, AGPT, AGP1, KIAA0003
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-400 of human ANGPT1 (NP_001186788.1).
NCBI Gene ID #:
284
NCBI Official Name:
angiopoietin 1
NCBI Official Symbol:
ANGPT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 51kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors
Swissprot #:
Q15389
User NOte:
Optimal dilutions for each application to be determined by the researcher.