CDH16 Antibody

ProSci
Product Code: PSI-23-251
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-23-251-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CDH16 antibody (23-251) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of rat kidney using CDH16 Polyclonal Antibody (23-251) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CDH16 antibody (23-251) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat kidney using CDH16 Polyclonal Antibody (23-251) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CDH16,
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-230 of human CDH16 (NP_004053.1).
NCBI Gene ID #:
1014
NCBI Official Name:
cadherin 16, KSP-cadherin
NCBI Official Symbol:
CDH16
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
O75309
User NOte:
Optimal dilutions for each application to be determined by the researcher.